Intergeneric hybridization in yeasts through protoplast fusion
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چکیده
منابع مشابه
Strain Improvement Through Protoplast Fusion for Enhanced Coffee Pulp Degradation
Pleurotus eous colonies regenerated on plates containing complete medium overlaid with soft agar gave 0.28% regeneration efficiency, while that of Pleurotus flabellatus was slightly less (0.24%). In PEG (polyethylene glycol) induced fusion experiments with Pleurotus eous and Pleurotus flabellatus, 0.18% of fusion frequency was achieved and four fusant colonies were recovered. The fusant No.4 co...
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In this study techniques for laboratory identification of dermatophyte fungi through protoplast hybridization were established. Firstly, auxotrophic mutants of different species of microsporum and trichophyton were induced and identified. Secondly, protoplasts from these mutants were isolated by digestion of their mycelium with Novozyme 234 using CaCI, (O.4M) as an osmotic stabilizer and g...
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As an alternative to sexual crossing, protoplasts from two strains of moss (Physcomitrella patens) can be hybridized using polyethylene glycol (PEG). Although the efficiency is low, it requires no sophisticated apparatus. Hybrids are readily obtained using complementary auxotrophic mutants or strains with transgenic antibiotic resistance markers. It is now routine to obtain hybrids using transg...
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De (Triticum aestivum): In vitro production and utilization of doubled haploids, p. 101–124. In: Y.P.S. Bajaj (ed.). Biotechnology in agriculture and forestry. vol. 12: Haploids in crop improvement I. Springer-Verlag, Berlin. Powell, W., P.D.S. Caligari, and J.M. Dunwell. 1986. Field performance of lines derived from haploid and diploid tissues of Hordeum vulgare. Theor. Applied Genet. 72:458–4...
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The subjects of this article are protoplast isolations and protoplast fusions, in particular their history, a review of factors influencing the protoplasts isolation and fusion, selection of hybrid plants and utilization of somatic hybrids in plant breeding. Somatic hybridization through protoplast fusion can overcome sexual incompatibility among plant species or genera; transfer genes of resis...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1978
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1978.tb01960.x